Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA.
WY Tarn, KR Lee, SC Cheng - Proceedings of the National …, 1993 - National Acad Sciences
WY Tarn, KR Lee, SC Cheng
Proceedings of the National Academy of Sciences, 1993•National Acad SciencesDuring assembly of the spliceosome, the U4 small nuclear RNA (snRNA) interacts with the
spliceosome as a preformed U4/U6-U5 triple small nuclear ribonucleoprotein (snRNP)
complex. Subsequently, U4 becomes loosely associated with the spliceosome, whereas U5
and U6 remain tightly associated, suggesting unwinding of the U4/U6 duplex. We show that
this step of the assembly process can be blocked by limiting the ATP concentration in the
splicing reaction. We also show that the yeast precursor mRNA processing protein PRP19 …
spliceosome as a preformed U4/U6-U5 triple small nuclear ribonucleoprotein (snRNP)
complex. Subsequently, U4 becomes loosely associated with the spliceosome, whereas U5
and U6 remain tightly associated, suggesting unwinding of the U4/U6 duplex. We show that
this step of the assembly process can be blocked by limiting the ATP concentration in the
splicing reaction. We also show that the yeast precursor mRNA processing protein PRP19 …
During assembly of the spliceosome, the U4 small nuclear RNA (snRNA) interacts with the spliceosome as a preformed U4/U6-U5 triple small nuclear ribonucleoprotein (snRNP) complex. Subsequently, U4 becomes loosely associated with the spliceosome, whereas U5 and U6 remain tightly associated, suggesting unwinding of the U4/U6 duplex. We show that this step of the assembly process can be blocked by limiting the ATP concentration in the splicing reaction. We also show that the yeast precursor mRNA processing protein PRP19 becomes associated with the spliceosome during this transition. Thus, PRP19 may function in this step of spliceosome assembly.
National Acad Sciences