An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation
To investigate the roles of intercellular gap junctions and extracellular ATP diffusion in bone
cell calcium signaling propagation in bone tissue, in vitro bone cell networks were
constructed by using microcontact printing and self-assembled monolayer technologies. In
the network, neighboring cells were interconnected through functional gap junctions. A
single cell at the center of the network was mechanically stimulated by using an AFM
nanoindenter. Intracellular calcium ([Ca2+] i) responses of the bone cell network were …
cell calcium signaling propagation in bone tissue, in vitro bone cell networks were
constructed by using microcontact printing and self-assembled monolayer technologies. In
the network, neighboring cells were interconnected through functional gap junctions. A
single cell at the center of the network was mechanically stimulated by using an AFM
nanoindenter. Intracellular calcium ([Ca2+] i) responses of the bone cell network were …