Identification and characterization of two distinct ligand binding regions of cubilin
RR Yammani, S Seetharam, B Seetharam - Journal of Biological Chemistry, 2001 - ASBMB
Using polymerase chain reaction-amplified fragments of cubilin, an endocytic receptor of
molecular mass 460 kDa, we have identified two distinct ligand binding regions. Region 1 of
molecular mass 71 kDa, which included the 113-residue N terminus along with the eight
epidermal growth factor (EGF)-like repeats and CUB domains 1 and 2, and region 2 of
molecular mass 37 kDa consisting of CUB domains 6–8 bound both intrinsic factor-
cobalamin (vitamin B 12; Cbl)(IF-Cbl) and albumin. Within these two regions, the binding of …
molecular mass 460 kDa, we have identified two distinct ligand binding regions. Region 1 of
molecular mass 71 kDa, which included the 113-residue N terminus along with the eight
epidermal growth factor (EGF)-like repeats and CUB domains 1 and 2, and region 2 of
molecular mass 37 kDa consisting of CUB domains 6–8 bound both intrinsic factor-
cobalamin (vitamin B 12; Cbl)(IF-Cbl) and albumin. Within these two regions, the binding of …
