Effects of restricted feeding on the activity of hypothalamic orexin (OX)-A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of rats
T Kurose, Y Ueta, Y Yamamoto, R Serino, Y Ozaki… - Regulatory peptides, 2002 - Elsevier
We have examined the effects of 3 weeks of food restriction on both the activity of neurons
containing hypothalamic orexin (OX)-A and the level of OX receptor type 2 (OX2R) mRNA in
the paraventricular nucleus (PVN) of rats. Double immunohistochemistry was used to
examine the expression of OX-A and Fos in the lateral hypothalamic area (LHA), and in situ
hybridization histochemistry was used to measure levels of OX2R mRNA in the PVN. After
the period of restricted feeding, 20–30% of OX-A-containing neurons exhibited Fos-like …
containing hypothalamic orexin (OX)-A and the level of OX receptor type 2 (OX2R) mRNA in
the paraventricular nucleus (PVN) of rats. Double immunohistochemistry was used to
examine the expression of OX-A and Fos in the lateral hypothalamic area (LHA), and in situ
hybridization histochemistry was used to measure levels of OX2R mRNA in the PVN. After
the period of restricted feeding, 20–30% of OX-A-containing neurons exhibited Fos-like …
