Human B Cell Development and Antibody Production in Humanized NOD/SCID/IL-2Rγnull (NSG) Mice Conditioned by Busulfan
Background Busulfan treatment as a chemotherapeutic agent has been considered an
alternative approach in xenograft model because it offers a simple, convenient, effective,
and less toxic conditioning regimen. Objective and methods To investigate busulfan effects
on the reconstitution of human immune cells and the generation of immune response to
foreign antigens, we generated humanized NOD/SCID/IL-2Rγ null (NSG) mice conditioned
either busulfan or total body irradiation (TBI) with hCD34+ CB cells. Results Busulfan …
alternative approach in xenograft model because it offers a simple, convenient, effective,
and less toxic conditioning regimen. Objective and methods To investigate busulfan effects
on the reconstitution of human immune cells and the generation of immune response to
foreign antigens, we generated humanized NOD/SCID/IL-2Rγ null (NSG) mice conditioned
either busulfan or total body irradiation (TBI) with hCD34+ CB cells. Results Busulfan …
Background
Busulfan treatment as a chemotherapeutic agent has been considered an alternative approach in xenograft model because it offers a simple, convenient, effective, and less toxic conditioning regimen.
Objective and methods
To investigate busulfan effects on the reconstitution of human immune cells and the generation of immune response to foreign antigens, we generated humanized NOD/SCID/IL-2Rγnull (NSG) mice conditioned either busulfan or total body irradiation (TBI) with hCD34+ CB cells.
Results
Busulfan resulted in a high survival rate and effective reconstitution of human immune cells including B, T, macrophage, and dendritic cells in humanized NSG mice, compared to that of TBI. Moreover, the humanized NSG mice conditioned busulfan showed effective B cell development and thereby the high production of human antibody against immunized antigen.
Conclusion
Humanized mice conditioned by busulfan provide a powerful and versatile tool for studying the entire process of human B-lymphocyte development and for producing specific human antibodies
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