Immunohistochemistry: paraffin sections using the Vectastain ABC kit from vector labs
V Chi, KG Chandy - JoVE (Journal of Visualized Experiments), 2007 - jove.com
Immunohistochemistry (IHC) is a valuable technique utilized to localize/visualize protein
expression in a mounted tissue section using specific antibodies. There are two methods:
the direct and indirect method. In this experiment, we will only describe the use of indirect
IHC staining. Indirect IHC staining utilizes highly specific primary and biotin-conjugated
secondary antibodies. Primary antibodies are utilized to discretely identify proteins of
interest by binding to a specific epitope, while secondary antibodies subtract for non-specific …
expression in a mounted tissue section using specific antibodies. There are two methods:
the direct and indirect method. In this experiment, we will only describe the use of indirect
IHC staining. Indirect IHC staining utilizes highly specific primary and biotin-conjugated
secondary antibodies. Primary antibodies are utilized to discretely identify proteins of
interest by binding to a specific epitope, while secondary antibodies subtract for non-specific …
