Imaging exocytosis of single insulin secretory granules with evanescent wave microscopy: DISTINCT BEHAVIOR OF GRANULE MOTION IN BIPHASIC INSULIN …
M Ohara-Imaizumi, Y Nakamichi, T Tanaka… - Journal of Biological …, 2002 - ASBMB
To study insulin exocytosis by monitoring the single insulin secretory granule motion,
evanescent wave microscopy was used to quantitatively analyze the final stage of insulin
exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in
MIN6 β cells was packed in insulin secretory granules, which appeared to preferentially
dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave
microscopy revealed that fluorescence of green fluorescent protein-tagged insulin …
evanescent wave microscopy was used to quantitatively analyze the final stage of insulin
exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in
MIN6 β cells was packed in insulin secretory granules, which appeared to preferentially
dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave
microscopy revealed that fluorescence of green fluorescent protein-tagged insulin …
