Dynamic-clamp analysis of wild-type human Nav1.7 and erythromelalgia mutant channel L858H

DV Vasylyev, C Han, P Zhao… - Journal of …, 2014 - journals.physiology.org
DV Vasylyev, C Han, P Zhao, S Dib-Hajj, SG Waxman
Journal of Neurophysiology, 2014journals.physiology.org
The link between sodium channel Nav1. 7 and pain has been strengthened by identification
of gain-of-function mutations in patients with inherited erythromelalgia (IEM), a genetic
model of neuropathic pain in humans. A firm mechanistic link to nociceptor dysfunction has
been precluded because assessments of the effect of the mutations on nociceptor function
have thus far depended on electrophysiological recordings from dorsal root ganglia (DRG)
neurons transfected with wild-type (WT) or mutant Nav1. 7 channels, which do not permit …
The link between sodium channel Nav1.7 and pain has been strengthened by identification of gain-of-function mutations in patients with inherited erythromelalgia (IEM), a genetic model of neuropathic pain in humans. A firm mechanistic link to nociceptor dysfunction has been precluded because assessments of the effect of the mutations on nociceptor function have thus far depended on electrophysiological recordings from dorsal root ganglia (DRG) neurons transfected with wild-type (WT) or mutant Nav1.7 channels, which do not permit accurate calibration of the level of Nav1.7 channel expression. Here, we report an analysis of the function of WT Nav1.7 and IEM L858H mutation within small DRG neurons using dynamic-clamp. We describe the functional relationship between current threshold for action potential generation and the level of WT Nav1.7 conductance in primary nociceptive neurons and demonstrate the basis for hyperexcitability at physiologically relevant levels of L858H channel conductance. We demonstrate that the L858H mutation, when modeled using dynamic-clamp at physiological levels within DRG neurons, produces a dramatically enhanced persistent current, resulting in 27-fold amplification of net sodium influx during subthreshold depolarizations and even greater amplification during interspike intervals, which provide a mechanistic basis for reduced current threshold and enhanced action potential firing probability. These results show, for the first time, a linear correlation between the level of Nav1.7 conductance and current threshold in DRG neurons. Our observations demonstrate changes in sodium influx that provide a mechanistic link between the altered biophysical properties of a mutant Nav1.7 channel and nociceptor hyperexcitability underlying the pain phenotype in IEM.
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