[HTML][HTML] Glyceraldehyde-3-phosphate Dehydrogenase Selectively Binds AU-rich RNA in the NAD+-binding Region (Rossmann Fold)(∗)
E Nagy, WFC Rigby - Journal of Biological Chemistry, 1995 - ASBMB
A 36-kDa protein that binds AU-rich RNA was purified from human spleen and identified as
glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GAPDH has been previously
demonstrated to bind tRNA with high affinity. Competition studies suggested that
cytoplasmic GAPDH binds the AU-rich elements (AREs) of lymphokine mRNA 3′-
untranslated regions with higher affinity than tRNA. The AUUUA-specific RNA binding
activity of GAPDH was inhibited by NAD+, NADH, and ATP in a concentration-dependent …
glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GAPDH has been previously
demonstrated to bind tRNA with high affinity. Competition studies suggested that
cytoplasmic GAPDH binds the AU-rich elements (AREs) of lymphokine mRNA 3′-
untranslated regions with higher affinity than tRNA. The AUUUA-specific RNA binding
activity of GAPDH was inhibited by NAD+, NADH, and ATP in a concentration-dependent …