A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells
Aids, 2014•journals.lww.com
We compared different techniques for measuring gut HIV reservoirs and assessed for HIV in
non-CD4+ T cells. HIV DNA levels were similar when measured from rectal biopsies and
isolated rectal cells, while HIV RNA tended to be higher in rectal cells. HIV DNA levels in
total rectal cells were greater than those predicted from levels in sorted CD4+ T cells,
suggesting a reservoir in non-CD4+ T cells, and HIV DNA was detected in sorted myeloid
cells (7/7 subjects).
non-CD4+ T cells. HIV DNA levels were similar when measured from rectal biopsies and
isolated rectal cells, while HIV RNA tended to be higher in rectal cells. HIV DNA levels in
total rectal cells were greater than those predicted from levels in sorted CD4+ T cells,
suggesting a reservoir in non-CD4+ T cells, and HIV DNA was detected in sorted myeloid
cells (7/7 subjects).
Abstract
We compared different techniques for measuring gut HIV reservoirs and assessed for HIV in non-CD4+ T cells. HIV DNA levels were similar when measured from rectal biopsies and isolated rectal cells, while HIV RNA tended to be higher in rectal cells. HIV DNA levels in total rectal cells were greater than those predicted from levels in sorted CD4+ T cells, suggesting a reservoir in non-CD4+ T cells, and HIV DNA was detected in sorted myeloid cells (7/7 subjects).
Lippincott Williams & Wilkins