Imaging of podocyte foot processes by fluorescence microscopy
I Grgic, CR Brooks, AF Hofmeister, V Bijol… - Journal of the …, 2012 - journals.lww.com
Visualizing podocyte foot processes requires electron microscopy, a technique that depends
on special equipment, requires immunogold for colabeling, and does not take advantage of
the growing number of in vivo fluorophores available. To address these limitations, we
developed a genetic strategy to allow detailed visualization of single podocytes and their
foot processes by conventional fluorescence microscopy. We generated a transgenic mouse
line expressing a GFP-Cre-ERT2 fusion protein under the control of the collagen α1 (I) …
on special equipment, requires immunogold for colabeling, and does not take advantage of
the growing number of in vivo fluorophores available. To address these limitations, we
developed a genetic strategy to allow detailed visualization of single podocytes and their
foot processes by conventional fluorescence microscopy. We generated a transgenic mouse
line expressing a GFP-Cre-ERT2 fusion protein under the control of the collagen α1 (I) …