The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant.
K Kornfeld, ML Reitman, R Kornfeld - Journal of Biological Chemistry, 1981 - ASBMB
The carbohydrate-binding specificities of pea lectin and lentil lectin have been determined
by testing the ability of radioactively labeled glycopeptides to bind to columns of pea lectin-
Sepharose and lentil lectin-Sepharose. The presence of a fucose residue attached to the
asparagine-linked N-acetylglucosamine residue of the test glycopeptide was essential for
high affinity binding to both pea and lentil lectin-Sepharose but not to concanavalin A-
Sepharose. In addition to fucose, 2 alpha-mannosyl residues were required for glycopeptide …
by testing the ability of radioactively labeled glycopeptides to bind to columns of pea lectin-
Sepharose and lentil lectin-Sepharose. The presence of a fucose residue attached to the
asparagine-linked N-acetylglucosamine residue of the test glycopeptide was essential for
high affinity binding to both pea and lentil lectin-Sepharose but not to concanavalin A-
Sepharose. In addition to fucose, 2 alpha-mannosyl residues were required for glycopeptide …
