[HTML][HTML] Adipocyte-selective reduction of the leptin receptors induced by antisense RNA leads to increased adiposity, dyslipidemia, and insulin resistance
JN Huan, J Li, Y Han, K Chen, N Wu… - Journal of Biological …, 2003 - ASBMB
Although recent evidence suggests that leptin can directly regulate a wide spectrum of
peripheral functions, including fat metabolism, genetic examples are still needed to illustrate
the physiological significance of direct actions of leptin in a given peripheral tissue. To this
end, we used a technical knock-out approach to reduce the expression of leptin receptors
specifically in white adipose tissue. The evaluation of leptin receptor reduction in adipocytes
was based on real time PCR analysis of the mRNA levels, Western blot analysis of the …
peripheral functions, including fat metabolism, genetic examples are still needed to illustrate
the physiological significance of direct actions of leptin in a given peripheral tissue. To this
end, we used a technical knock-out approach to reduce the expression of leptin receptors
specifically in white adipose tissue. The evaluation of leptin receptor reduction in adipocytes
was based on real time PCR analysis of the mRNA levels, Western blot analysis of the …
