[HTML][HTML] Architecture of Ca2+ channel pore-lining segments revealed by covalent modification of substituted cysteines
SE Koch, I Bodi, A Schwartz, G Varadi - Journal of Biological Chemistry, 2000 - ASBMB
The cysteine accessibility method was used to explore calcium channel pore topology.
Cysteine mutations were introduced into the SS1-SS2 segments of Motifs I-IV of the human
cardiac L-type calcium channel, expressed in Xenopusoocytes and the current block by
methanethiosulfonate compounds was measured. Our studies revealed that several
consecutive mutants of motifs II and III are accessible to methanethiosulfonates, suggesting
that these segments exist as random coils. Motif I cysteine mutants exhibited an intermittent …
Cysteine mutations were introduced into the SS1-SS2 segments of Motifs I-IV of the human
cardiac L-type calcium channel, expressed in Xenopusoocytes and the current block by
methanethiosulfonate compounds was measured. Our studies revealed that several
consecutive mutants of motifs II and III are accessible to methanethiosulfonates, suggesting
that these segments exist as random coils. Motif I cysteine mutants exhibited an intermittent …