Isolation, characterization, and differentiation of human embryonic stem cells
MF Pera, AA Filipczyk, SM Hawes, AL Laslett - Methods in enzymology, 2003 - Elsevier
Publisher Summary The first derivation of human embryonic stem (ES) cells was reported in
1998, and although there have been a number of anecdotal reports of isolation of new ES
cells since then, published data are based only on a few cell lines. Timely progress in this
field depends upon the derivation of new ES cell lines, their proper characterization and
comparison with existing isolates, and in-depth analysis of their differentiation under
different conditions. Previous reviews have compared the properties of published human ES …
1998, and although there have been a number of anecdotal reports of isolation of new ES
cells since then, published data are based only on a few cell lines. Timely progress in this
field depends upon the derivation of new ES cell lines, their proper characterization and
comparison with existing isolates, and in-depth analysis of their differentiation under
different conditions. Previous reviews have compared the properties of published human ES …