[HTML][HTML] Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice
M TeKippe, DE Harrison, J Chen - Experimental hematology, 2003 - Elsevier
Objectives. To study the effects of transformation-related protein 53 (Trp53) and other genes
on hematopoiesis and hematopoietic stem cells (HSCs). Methods. Frequencies of murine
bone marrow cells (BMCs) with the Lin− Sca-1+ c-kit+ CD34− phenotype were analyzed by
flow cytometry, and were increased in mice with germ-line deletion of the Trp53 (Trp53−/−)
gene but not in 25 other deletions of genes involved in cell cycling, development, cancer, or
hematopoiesis. Therefore, Trp53−/− and wild-type Trp53+/+ mice were compared using the …
on hematopoiesis and hematopoietic stem cells (HSCs). Methods. Frequencies of murine
bone marrow cells (BMCs) with the Lin− Sca-1+ c-kit+ CD34− phenotype were analyzed by
flow cytometry, and were increased in mice with germ-line deletion of the Trp53 (Trp53−/−)
gene but not in 25 other deletions of genes involved in cell cycling, development, cancer, or
hematopoiesis. Therefore, Trp53−/− and wild-type Trp53+/+ mice were compared using the …
