Association of increased autophagic inclusions labeled for β-galactosidase with fibroblastic aging
LM Gerland, S Peyrol, C Lallemand, R Branche… - Experimental …, 2003 - Elsevier
Replicative senescence appears after a finite number of cell divisions. After proliferation has
ceased, senescent cells remain viable for long periods and metabolic modifications are
observed such as lipofuscin accumulation. In order to understand this phenomenon, we
examined the emergence of subcellular modifications corresponding to autophagy in MRC5
normal human fibroblasts. An increase of monodansylcadaverine fluorescence, a specific
marker of autophagy, in aging compared to young fibroblasts was observed (p< 0.0001) …
ceased, senescent cells remain viable for long periods and metabolic modifications are
observed such as lipofuscin accumulation. In order to understand this phenomenon, we
examined the emergence of subcellular modifications corresponding to autophagy in MRC5
normal human fibroblasts. An increase of monodansylcadaverine fluorescence, a specific
marker of autophagy, in aging compared to young fibroblasts was observed (p< 0.0001) …
