Differential effects of histone deacetylase inhibitors on interleukin-18 gene expression in myeloid cells
N Koyama, S Koschmieder, S Tyagi… - Biochemical and …, 2002 - Elsevier
N Koyama, S Koschmieder, S Tyagi, H Nürnberger, S Wagner, U Böcker, D Hoelzer…
Biochemical and biophysical research communications, 2002•ElsevierHistone deacetyrase (HDAC) inhibitors induce growth arrest and differentiation of leukemia
cell lines and tumor cells derived from a large variety of human tissues. Here we showed
that HDAC inhibitors sodium butyrate, TSA, and valproate regulated the expression of
Interleukin-18 (IL-18), a cytokine with antitumor and proinflammatory properties, in human
acute myeloid leukemia cell lines U937 and HEL. Sodium butyrate increased expression of
IL-18 protein and mRNA and activated 1357bp IL-18 gene promoter construct. IL-18 mRNA …
cell lines and tumor cells derived from a large variety of human tissues. Here we showed
that HDAC inhibitors sodium butyrate, TSA, and valproate regulated the expression of
Interleukin-18 (IL-18), a cytokine with antitumor and proinflammatory properties, in human
acute myeloid leukemia cell lines U937 and HEL. Sodium butyrate increased expression of
IL-18 protein and mRNA and activated 1357bp IL-18 gene promoter construct. IL-18 mRNA …
Histone deacetyrase (HDAC) inhibitors induce growth arrest and differentiation of leukemia cell lines and tumor cells derived from a large variety of human tissues. Here we showed that HDAC inhibitors sodium butyrate, TSA, and valproate regulated the expression of Interleukin-18 (IL-18), a cytokine with antitumor and proinflammatory properties, in human acute myeloid leukemia cell lines U937 and HEL. Sodium butyrate increased expression of IL-18 protein and mRNA and activated 1357bp IL-18 gene promoter construct. IL-18 mRNA level was up-regulated by TSA or valproate, which also activated IL-18 full-length promoter. While sodium butyrate or TSA stimulated the 108-bp IL-18 minimal promoter, valproate failed to activate it, indicating that valproate may use a distinct mechanism from sodium butyrate and TSA to activate IL-18 gene expression.
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