Isotopic labeling of DNA in rat adipose tissue: evidence for proliferating cells associated with mature adipocytes.
BJ Klyde, J Hirsch - Journal of lipid research, 1979 - ASBMB
The intraperitoneal administration of [3H] thymidine to adult rats resulted in the rapid
appearance of label in the adipocyte fraction of collagenase digests of adipose tissue. Low-
speed centrifugation followed by freezing and slicing showed the label to be uniformly
distributed in the adipocyte fraction. The presence of label in DNA was confirmed by
hydrolysis with deoxyribonuclease and by inhibition of incorporation with hydroxyurea.
Organelle fractionation revealed that the label was predominantly in nuclei, and …
appearance of label in the adipocyte fraction of collagenase digests of adipose tissue. Low-
speed centrifugation followed by freezing and slicing showed the label to be uniformly
distributed in the adipocyte fraction. The presence of label in DNA was confirmed by
hydrolysis with deoxyribonuclease and by inhibition of incorporation with hydroxyurea.
Organelle fractionation revealed that the label was predominantly in nuclei, and …
