[HTML][HTML] Role of bound zinc in dimer stabilization but not enzyme activity of neuronal nitric-oxide synthase
B Hemmens, W Goessler, K Schmidt… - Journal of Biological …, 2000 - ASBMB
Nitric-oxide synthases (NOS) are homodimeric proteins and can form an intersubunit Zn (4S)
cluster. We have measured zinc bound to NOS purified from pig brain (0.6 mol/mol of NOS)
and baculovirus-expressed rat neuronal NOS (nNOS)(0.49±0.13 mol/mol of NOS), by on-
line gel-filtration/inductively coupled plasma mass spectrometry. Cobalt, manganese,
molybdenum, nickel, and vanadium were all undetectable. Baculovirus-expressed nNOS
also bound up to 2.00±0.58 mol of copper/mol of NOS. Diethylenetriaminepentaacetic acid …
cluster. We have measured zinc bound to NOS purified from pig brain (0.6 mol/mol of NOS)
and baculovirus-expressed rat neuronal NOS (nNOS)(0.49±0.13 mol/mol of NOS), by on-
line gel-filtration/inductively coupled plasma mass spectrometry. Cobalt, manganese,
molybdenum, nickel, and vanadium were all undetectable. Baculovirus-expressed nNOS
also bound up to 2.00±0.58 mol of copper/mol of NOS. Diethylenetriaminepentaacetic acid …
